PIM2 Mouse Monoclonal Antibody [Clone ID: OTI5D5]

CAT#: CF501063

Carrier-free (BSA/glycerol-free) PIM2 mouse monoclonal antibody, clone OTI5D5 (formerly 5D5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (RC201933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2. Positive lysates LY416355 (100ug) and LC416355 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501063)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501063)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501063)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501063)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501063)

• HEK293T cells transfected with either RC201933 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIM2 antibody (TA501063), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-PIM2 antibody (TA501063), (Red), compared to a nonspecific negative control antibody, (Blue).

• Immunoprecipitation (IP) of PIM2 by using TrueMab monoclonal anti-PIM2 antibodies (Negative control: IP without adding anti-PIM2 antibody.). For each experiment, 500ul of DDK tagged PIM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PIM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• Anti-PIM2 mouse monoclonal antibody (TA501063) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2 (RC201933).

• Immunofluorescent staining of HT29 cells using anti-PIM2 mouse monoclonal antibody (TA501063).

• Immunofluorescent staining of HeLa cells using anti-PIM2 mouse monoclonal antibody (TA501063).

• Western blot analysis of extracts (10ug) from 4 human tissue by using anti-PIM2 monoclonal antibody at 1:200 dilution.

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Specifications

Product Data

• Clone Name: OTI5D5
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34 kDa
• Gene Name: Homo sapiens Pim-2 proto-oncogene, serine/threonine kinase (PIM2), mRNA.
• Database Link:
  NP_006866
  Entrez Gene 18715 MouseEntrez Gene 317366 RatEntrez Gene 11040 Human
  UniProt ID Q9P1W9
• Background: This gene encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia