PTP epsilon (PTPRE) Mouse Monoclonal Antibody [Clone ID: OTI3D4]

CAT#: CF501051

Carrier-free (BSA/glycerol-free) PTPRE mouse monoclonal antibody, clone OTI3D4 (formerly 3D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRE (RC207950, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRE. Positive lysates LY401950 (100ug) and LC401950 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Anti-PTPRE mouse monoclonal antibody (TA501051) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE (RC207950).

• Immunofluorescent staining of HT29 cells using anti-PTPRE mouse monoclonal antibody (TA501051).

• Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-PTPRE monoclonal antibody at 1:200 dilution.

• Flow cytometric Analysis of living Malme-3M cells, using anti-PTPRE antibody (TA501051), (Red), compared to PBS, (Blue).

Show all 12 images

Specifications

Product Data

• Clone Name: OTI3D4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:50
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PTPRE (NP_006495) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80.5 kDa
• Gene Name: protein tyrosine phosphatase receptor type E
• Database Link:
  NP_006495
  Entrez Gene 19267 MouseEntrez Gene 114767 RatEntrez Gene 5791 Human
  UniProt ID P23469
• Background: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Two alternatively spliced transcript variants of this gene have been reported, one of which encodes a receptor-type PTP that possesses a short extracellular domain, a single transmembrane region, and two tandem intracytoplasmic catalytic domains; Another one encodes a PTP that contains a distinct hydrophilic N-terminus, and thus represents a nonreceptor-type isoform of this PTP. Studies of the similar gene in mice suggested the regulatory roles of this PTP in RAS related signal transduction pathways, cytokines induced SATA signaling, as well as the activation of voltage-gated K+ channels. [provided by RefSeq, Jul 2008]
• Synonyms: HPTPE; PTPE; R-PTP-EPSILON

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Transmembrane