MCL1 Mouse Monoclonal Antibody [Clone ID: OTI10F6]

CAT#: CF501036

Carrier-free (BSA/glycerol-free) MCL1 mouse monoclonal antibody, clone OTI10F6 (formerly 10F6)

Product Images

HEK293T cells transfected with either pCMV6-ENTRY MCL1 (RC200521) (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal (TA501036), and then analyzed by flow cytometry.

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  Anti-MCL1 mouse monoclonal antibody (TA501036) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1 (RC200521).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (RC200521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1. Positive lysates LY411855 (100ug) and LC411855 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501036, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501036, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501036, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501036, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501036, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501036, Dilution 1:50)

• Western blot analysis of extracts (10ug) from 1 cell line by using anti-MCL1 monoclonal antibody at 1:200.

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Specifications

Product Data

• Clone Name: OTI10F6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.3 kDa
• Gene Name: MCL1 apoptosis regulator, BCL2 family member
• Database Link:
  NP_068779
  Entrez Gene 4170 Human
  UniProt ID Q07820
• Background: The protein encoded by this gene belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.
• Synonyms: bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM

Reference Data

• Protein Families: Druggable Genome, Transmembrane