RPA34 (RPA2) Mouse Monoclonal Antibody [Clone ID: OTI1H10]

CAT#: CF500765

Carrier-free (BSA/glycerol-free) RPA2 mouse monoclonal antibody, clone OTI1H10 (formerly 1H10)

Product Images

Anti-RPA2 mouse monoclonal antibody (TA500765) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2 (RC205715).

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  Immunoprecipitation (IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500765)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500765)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500765)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500765)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500765)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500765)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500765)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500765)

• Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500765)

• HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2. Positive lysates LY401031 (100ug) and LC401031 (20ug) can be purchased separately from OriGene.

• Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• HEK293T cells transfected with either RC205715 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RPA2 antibody (TA500765), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-RPA2 antibody (TA500765), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody (TA500765), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1H10
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RPA2 (NP_002937) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.1 kDa
• Gene Name: replication protein A2
• Database Link:
  NP_002937
  Entrez Gene 19891 MouseEntrez Gene 59102 RatEntrez Gene 100856421 DogEntrez Gene 716612 MonkeyEntrez Gene 6118 Human
  UniProt ID P15927
• Synonyms: REPA2; RP-A p32; RP-A p34; RPA32

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Homologous recombination, Mismatch repair, Nucleotide excision repair