BCKDH kinase (BCKDK) Mouse Monoclonal Antibody [Clone ID: OTI11C9]

SKU CF500685

Carrier-free (BSA/glycerol-free) BCKDK mouse monoclonal antibody, clone OTI11C9 (formerly 11C9)

Product Data

• Clone Name: OTI11C9
• Application: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BCKDK (NP_005872) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 46.4 kDa
• Gene Name: branched chain keto acid dehydrogenase kinase
• Database Link:
  NP_005872
  O14874
• Background: Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex,the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex .
• Synonyms: BCKDKD; BDK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase