XRCC4 Mouse Monoclonal Antibody [Clone ID: OTI4H9]

CAT#: CF500650

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Carrier-free (BSA/glycerol-free) XRCC4 mouse monoclonal antibody, clone OTI4H9 (formerly 4H9)



  View other "OTI4H9" antibodies (4)

USD 600.00


Availability*
In Stock

Size
    • 100 ug


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Specifications

Product Data
Clone Name OTI4H9
Applications IHC, WB
Recommended Dilution WB 1:2000, IHC 1:50
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full-length protein expressed in 293T cell transfected with human XRCC4 expression vector
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 38.3 kDa
Gene Name Homo sapiens X-ray repair cross complementing 4 (XRCC4), transcript variant 3, mRNA.
Background The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants.
Synonyms SSMED
Reference Data
Protein Families Druggable Genome
Protein Pathways Non-homologous end-joining

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