Protein kinase Y linked (PRKY) Mouse Monoclonal Antibody [Clone ID: OTI3E10]

SKU CF500569

Carrier-free (BSA/glycerol-free) PRKY mouse monoclonal antibody, clone OTI3E10 (formerly 3E10)

Product Data

• Clone Name: OTI3E10
• Application: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human PRKY expression vector
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 31.7 kDa
• Gene Name: protein kinase Y-linked (pseudogene)
• Database Link:
  NP_002751
  O43930
• Background: This gene is similar to the protein kinase, X-linked gene in the pseudoautosomal region of the X chromsoome. The gene is classified as a transcribed pseudogene because it has lost a coding exon that results in all transcripts being candidates for nonsense-mediated decay (NMD) and unlikely to express a protein.Abnormal recombination between this gene and a related gene on chromosome X is a frequent cause of XX males and XY females.
• Synonyms: OTTHUMP00000033227; protein kinase, Y-linked

Reference Data

• Protein Families: Druggable Genome, Protein Kinase