Glucose Transporter 5 GLUT5 (SLC2A5) Mouse Monoclonal Antibody [Clone ID: OTI9F3]

CAT#: CF500556

Carrier-free (BSA/glycerol-free) SLC2A5 mouse monoclonal antibody, clone OTI9F3 (formerly 9F3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SLC2A5 (Cat# RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A5(Cat# TA500556). Positive lysates LY401061 (100ug) and LC401061 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SLC2A5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunoprecipitation (IP) of SLC2A5 by using TrueMab monoclonal anti-SLC2A5 antibodies (Negative control: IP without adding anti-SLC2A5 antibody.). For each experiment, 500ul of DDK tagged SLC2A5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC2A5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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  Flow cytometric Analysis of Hela cells, using anti-SLC2A5 antibody (TA500556), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-SLC2A5 antibody (TA500556), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI9F3
• Applications: FC, IHC, IP, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:50, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.8 kDa
• Gene Name: solute carrier family 2 member 5
• Database Link:
  NP_003030
  Entrez Gene 722154 MonkeyEntrez Gene 6518 Human
  UniProt ID P22732
• Synonyms: GLUT-5; GLUT5

Reference Data

• Protein Families: Transmembrane