NY-ESO-1 (CTAG1B) Mouse Monoclonal Antibody [Clone ID: OTI2B6]

CAT#: CF500528

Carrier-free (BSA/glycerol-free) CTAG1B (NY-ESO-1) mouse monoclonal antibody, clone OTI2B6 (formerly 2B6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CTAG1B (Cat# RC213318, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B(Cat# TA500528). Positive lysates LY400527 (100ug) and LC400527 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CTAG1B monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500528, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CTAG1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500528, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-CTAG1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500528, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-CTAG1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500528, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CTAG1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500528, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-CTAG1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500528, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-CTAG1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500528, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-CTAG1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500528, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-CTAG1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500528, Dilution 1:50)

• Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY CTAG1B (RC213318) using anti-CTAG1B antibody (TA500528/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).

• Flow cytometric analysis of living 293T cells transfected with CTAG1B overexpression plasmid (RC213318), Red)/empty vector (PS100001, Blue) using anti-CTAG1B antibody (TA500528). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

• Flow cytometric analysis of living NCI-H1299 cells, using anti-CTAG1B antibody (TA500528, Red), compared to an isotype control (green) (1:100).

• Flow cytometric analysis of living A375 cells, using anti-CTAG1B antibody (TA500528, Red), compared to an isotype control (green) (1:100).

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Specifications

Product Data

• Clone Name: OTI2B6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:5000 IHC 1:50 IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human CTAG1B expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18 kDa
• Gene Name: cancer/testis antigen 1B
• Database Link:
  NP_001318
  Entrez Gene 1485 Human
  UniProt ID P78358
• Background: Cancer/testis antigens, such as CTAG1B, are expressed in a variety of malignant tumors but soley in testis among normal adult tissues (Yoshida et al., 2006 (PubMed 16596224)).
• Synonyms: CT6.1; CTAG; CTAG1; ESO1; LAGE-2; LAGE2B; NY-ESO-1

Reference Data

• Protein Families: Druggable Genome