SATB1 Mouse Monoclonal Antibody [Clone ID: OTI13D6]

CAT#: CF500522

Carrier-free (BSA/glycerol-free) SATB1 mouse monoclonal antibody, clone OTI13D6 (formerly 13D6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SATB1 (Cat# RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1(Cat# TA500522). Positive lysates LY418976 (100ug) and LC418976 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SATB1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500522, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500522, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500522, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500522, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500522, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500522, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500522, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500522, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500522, Dilution 1:50)

• Anti-SATB1 mouse monoclonal antibody (TA500522) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1 (RC200421).

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Specifications

Product Data

• Clone Name: OTI13D6
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000 IHC 1:50 IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human SATB1 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 86 kDa
• Gene Name: Homo sapiens SATB homeobox 1 (SATB1), transcript variant 1, mRNA.
• Database Link:
  NP_002962
  Entrez Gene 20230 MouseEntrez Gene 316164 RatEntrez Gene 6304 Human
  UniProt ID Q01826
• Background: The protein binds to DNA at special AT-rich sequences at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA.
• Synonyms: DNA-binding protein SATB1; SATB homeobox 1; scaffold-associating DNA's); special AT-rich sequence binding protein 1; special AT-rich sequence binding protein 1 (binds to nuclear matrix

Reference Data

• Protein Families: Druggable Genome, Transcription Factors