Melanoma gp100 (PMEL) Mouse Monoclonal Antibody [Clone ID: OTI8A4]

CAT#: CF500402

Carrier-free (BSA/glycerol-free) SILV mouse monoclonal antibody, clone OTI8A4 (formerly 8A4)

Formulation: Standard Carrier-Free


  View other "OTI8A4" antibodies (4)

USD 600.00

4 Weeks*

Size
    • 100 ug

Frequently bought together (1)
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    • 200 ug

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Specifications

Product Data
Clone Name OTI8A4
Applications IF, IHC, WB
Recommended Dilution WB 1:1000, IHC: 1:150
Reactivities Human, Dog, Rat, Monkey
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Full-length protein expressed in 293T cell transfected with human SILV expression vector
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 70.3 kDa
Gene Name Homo sapiens premelanosome protein (PMEL), transcript variant 3, mRNA.
Background Could be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity
Synonyms D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV
Reference Data
Protein Families Secreted Protein, Transmembrane

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.