OXSR1 Mouse Monoclonal Antibody [Clone ID: OTI1F3]

CAT#: CF500392

Carrier-free (BSA/glycerol-free) OXSR1 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY OXSR1 (Cat# RC200396, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OXSR1(Cat# TA500392). Positive lysates LY401573 (100ug) and LC401573 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OXSR1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-OXSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500392)

Specifications

Product Data

• Clone Name: OTI1F3
• Applications: IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human OXSR1 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.8 kDa
• Gene Name: Homo sapiens oxidative stress responsive kinase 1 (OXSR1), mRNA.
• Database Link:
  NP_005100
  Entrez Gene 108737 MouseEntrez Gene 316064 RatEntrez Gene 607809 DogEntrez Gene 696362 MonkeyEntrez Gene 9943 Human
  UniProt ID O95747
• Background: The product of this gene belongs to the Ser/Thr protein kinase family of proteins. It regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton.Regulates downstream kinases in response to environmental stress. May also have a function in regulating the actin cytoskeleton
• Synonyms: OSR1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase