Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: OTI3F8]

SKU CF500212

Carrier-free (BSA/glycerol-free) CK19 mouse monoclonal antibody, clone OTI3F8 (formerly 3F8)

Product Data

• Clone Name: OTI3F8
• Application: IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50 - 1:150, IF 1:100
• Reactivity: Dog, Human, Monkey, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 43.9 kDa
• Gene Name: keratin 19
• Database Link:
  NP_002267
  P08727
• Background: Keratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.
• Synonyms: CK19; K1CS; K19

Reference Data