STAT3 Mouse Monoclonal Antibody [Clone ID: OTI21E7]

CAT#: CF500177

Carrier-free (BSA/glycerol-free) STAT3 mouse monoclonal antibody, clone OTI21E7 (formerly 21E7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STAT3 (Cat# RC212394, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT3(Cat# TA500177). Positive lysates LY403728 (100ug) and LC403728 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from different cell line by using anti-STAT3 monoclonal antibody (1:500).

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  Western blot analysis of extracts (35ug) from different cell line by using anti-STAT3 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and STAT3-Knockout Hela cells (KO, Cat# LC810228) were separated by SDS-PAGE and immunoblotted with anti-STAT3 monoclonal antibody TA500177. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).

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  Immunohistochemical staining of paraffin-embedded breast within the normal limits using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

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  Immunohistochemical staining of paraffin-embedded colon within the normal limits using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

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  Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

• Immunohistochemical staining of paraffin-embedded Carcinoma of kidney using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

• Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

• Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

• Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

• Immunohistochemical staining of paraffin-embedded thyroid within the normal limits using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

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Specifications

Product Data

• Clone Name: OTI21E7
• Applications: IHC, WB
• Recommended Dilution: WB: 1:500, IHC 1:50
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 154-175 of human stat3
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 88.08 kDa
• Gene Name: Homo sapiens signal transducer and activator of transcription 3 (STAT3), transcript variant 3, mRNA.
• Database Link:
  NP_998827
  Entrez Gene 20848 MouseEntrez Gene 25125 RatEntrez Gene 6774 Human
  UniProt ID P40763
• Background: The protein encoded by this gene is a member of the STAT protein family. In response to cytokinesand growth factors, STAT family members are phosphorylated by the receptor associated kinases, andthen form homo- or heterodimers that translocate to the cell nucleus where they act astranscription activators. This protein is activated through phosphorylation in response to variouscytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This proteinmediates the expression of a variety of genes in response to cell stimuli, and thus plays a keyrole in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has beenshown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor ofthis protein. Three alternatively spliced transcript variants encoding distinct isoforms have beendescribed
• Synonyms: ADMIO; APRF; HIES

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Chemokine signaling pathway, Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer