STAT3 Mouse Monoclonal Antibody [Clone ID: OTI21E7]

SKU CF500177

Carrier-free (BSA/glycerol-free) STAT3 mouse monoclonal antibody, clone OTI21E7 (formerly 21E7)

Product Data

• Clone Name: OTI21E7
• Application: IHC, WB
• Recommended Dilution: WB: 1:500, IHC 1:50
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 154-175 of human stat3
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 88.08 kDa
• Gene Name: signal transducer and activator of transcription 3
• Database Link:
  NP_998827
  P40763
• Background: The protein encoded by this gene is a member of the STAT protein family. In response to cytokinesand growth factors, STAT family members are phosphorylated by the receptor associated kinases, andthen form homo- or heterodimers that translocate to the cell nucleus where they act astranscription activators. This protein is activated through phosphorylation in response to variouscytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This proteinmediates the expression of a variety of genes in response to cell stimuli, and thus plays a keyrole in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has beenshown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor ofthis protein. Three alternatively spliced transcript variants encoding distinct isoforms have beendescribed
• Synonyms: ADMIO; APRF; HIES

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Chemokine signaling pathway, Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer