Neurotrophin 3 (NTF3) Mouse Monoclonal Antibody [Clone ID: OTI5C7]

CAT#: CF500115

Carrier-free (BSA/glycerol-free) NT3 mouse monoclonal antibody, clone OTI5C7 (formerly 5C7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NTF3 (Cat# RC214281, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTF3(Cat# TA500115). Positive lysates LY420181 (100ug) and LC420181 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of recombinant human NTF3 protein (Tyr139-Thr257, 0.02ug) by using anti-NTF3 antibody (TA500115). (1:500)

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  Immunohistochemical staining of paraffin-embedded breast within the normal limits using anti-NTF3 (TA500115) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast using anti-NTF3 (TA500115) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon using anti-NTF3 (TA500115) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

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  Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-NTF3 (TA500115) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded Carcinoma of kidney using anti-NTF3 (TA500115) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded liver within the normal limits using anti-NTF3 (TA500115) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-NTF3 (TA500115) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-NTF3 (TA500115) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-NTF3 (TA500115) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary using anti-NTF3 (TA500115) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-NTF3 (TA500115) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid using anti-NTF3 (TA500115) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded endometrium within the normal limits using anti-NTF3 (TA500115) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Figure from citation: Detection of NT-3 (NTF3) protein in brain metastases from breast cancer patients. (a) × 100 magnification of the brain metastasis tissue section with the isotype-matched negative control antibody staining. (b) ×100 magnification of the brain metastasis tissue section with the NT-3 antibody staining. View Citation

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Specifications

Product Data

• Clone Name: OTI5C7
• Applications: IHC, WB
• Recommended Dilution: WB: 1:5000, IHC 1:50
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 139-257 of human NT3
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.4 kDa
• Gene Name: Homo sapiens neurotrophin 3 (NTF3), transcript variant 1, mRNA.
• Database Link:
  NP_001096124
  Entrez Gene 18205 MouseEntrez Gene 81737 RatEntrez Gene 4908 Human
  UniProt ID P20783
• Background: NT3 is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. It is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse
• Synonyms: HDNF; NGF-2; NGF2; NT-3; NT3

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Neurotrophin signaling pathway