HES1 Mouse Monoclonal Antibody [Clone ID: OTI4H1]

CAT#: CF500114

Carrier-free (BSA/glycerol-free) HES1 mouse monoclonal antibody, clone OTI4H1 (formerly 4H1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (RC211709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1 (1:500).

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  Immunofluorescent staining of HeLa cells using anti-HES1 mouse monoclonal antibody (TA500114).

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  Anti-HES1 mouse monoclonal antibody (TA500114) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HES1 (RC211709).

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  Immunohistochemical staining of paraffin-embedded endometrium within the normal limits using anti-HES1 (TA500114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-HES1 (TA500114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

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  Immunohistochemical staining of paraffin-embedded liver within the normal limits using anti-HES1 (TA500114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-HES1 (TA500114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded breast within the normal limits using anti-HES1 ([TA000114]) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

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Specifications

Product Data

• Clone Name: OTI4H1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000; IHC 1:50; RPPA 1:75; IF (1:100)
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 1-280 of human HES1
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.4 kDa
• Gene Name: Homo sapiens hes family bHLH transcription factor 1 (HES1), mRNA.
• Database Link:
  NP_005515
  Entrez Gene 15205 MouseEntrez Gene 29577 RatEntrez Gene 3280 Human
  UniProt ID Q14469
• Background: This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
• Synonyms: bHLHb39; HES-1; HHL; HRY

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young, Notch signaling pathway