GATA4 Mouse Monoclonal Antibody [Clone ID: OTI9F9]

SKU CF500091

Carrier-free (BSA/glycerol-free) GATA4 mouse monoclonal antibody, clone OTI9F9 (formerly 9F9)

Product Data

• Clone Name: OTI9F9
• Application: IF, IHC, WB
• Recommended Dilution: WB 1:200~500, IHC 1:50, IF 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 296-442 of human GATA4 (NP_002043) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 44.4 kDa
• Gene Name: GATA binding protein 4
• Database Link:
  NP_002043
  P43694
• Background: GATA4 encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.
• Synonyms: ASD2; TACHD; TOF; VSD1

Reference Data

• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors