Neurotrophin 4 (NTF4) Mouse Monoclonal Antibody [Clone ID: OTI2E8]

CAT#: CF500034

Carrier-free (BSA/glycerol-free) NT4 mouse monoclonal antibody, clone OTI2E8 (formerly 2E8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NTF4 (Cat# RC204535, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTF4(Cat# TA500034). Positive lysates LY416817 (100ug) and LC416817 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-NTF4 monoclonal antibody at 1:200 dilution.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NTF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500034)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NTF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500034)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NTF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500034)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NTF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500034)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NTF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500034)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NTF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500034)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NTF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500034)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NTF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500034)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-NTF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500034)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NTF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500034)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NTF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500034)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NTF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500034)

• Immunofluorescent staining of A549 cells using anti-NTF4 mouse monoclonal antibody (TA500034).

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Specifications

Product Data

• Clone Name: OTI2E8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:5000, IHC 1:50, IF 1:50
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 81-210 human NT4 (NP_006170) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.2 kDa
• Gene Name: Homo sapiens neurotrophin 4 (NTF4), mRNA.
• Database Link:
  NP_006170
  Entrez Gene 25730 RatEntrez Gene 4909 Human
  UniProt ID P34130
• Background: NT4 is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of NT4 is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NT4-deficient mice only show minor cellular deficits and develop normally to adulthood.
• Synonyms: GLC1O; GLC10; NT-4; NT-4/5; NT-5; NT4; NT5; NTF5

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Neurotrophin signaling pathway