c-Myc (MYC) Mouse Monoclonal Antibody [Clone ID: OTI5G3]

CAT#: CF500004

Carrier-free (BSA/glycerol-free) c-Myc mouse monoclonal antibody, clone OTI5G3 (formerly 5G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYC (RC201611, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYC. Positive lysates LY400876 (100ug) and LC400876 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MYC monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500004)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500004)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500004)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500004)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500004)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500004)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500004)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500004)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500004)

• Anti-MYC mouse monoclonal antibody (TA500004) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MYC (RC201611).

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Specifications

Product Data

• Clone Name: OTI5G3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:50~100, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human Myc (NP_002458) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.4 kDa
• Gene Name: MYC proto-oncogene, bHLH transcription factor
• Database Link:
  NP_002458
  Entrez Gene 17869 MouseEntrez Gene 24577 RatEntrez Gene 4609 Human
  UniProt ID P01106
• Background: c-Myc is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
• Synonyms: bHLHe39; c-Myc; MRTL; MYCC

Reference Data

• Protein Families: Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway, Thyroid cancer, Wnt signaling pathway