Collagen type I Rabbit Polyclonal Antibody

CAT#: BP8001S

Collagen type I rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: ELISA.
  RIA: 1/1000.
  Immunfluorescence: 1/40-1/80.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 1/500-1/1000.
  Incubation time: 60 min at RT or 2-8°C overnight.
  Positive Control: Chicken skin.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Chicken
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified collagen type I from chicken skin
• Specificity: Chicken Collagen type I: 100%.
  Chicken Collagen type III and IV: < 0.1 %.
  Collagen type I Human, Mouse, Rat, Cattle: < 0.1 %.
  Chicken Fibronectin: < 0.1 % RIA at 1/1000 dilution.
• Formulation: State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore by adding 0.1 ml aqua destilled, for further dilution use appropriate antibody diluent.
• Concentration: lot specific
• Purification: Ion Exchange Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P02457
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
• Synonyms: COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen