Cytokeratin 8 (KRT8) Guinea Pig Polyclonal Antibody

CAT#: BP5075

Cytokeratin 8 (KRT8) guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting: 1/3000 (ECL method).
  Immunohistochemistry on Frozen Sections: 1/50 - 1/250.
  Immunohistochemistry on Paraffin Sections: 1/50 - 1/250 (after microwave treatment or trypsin).
  Immunohistochemistry on Cytological Material.
  Incubation Time: 1 h at RT, extended with paraffin sections.
  Positive Control: Human intestinal epithelia.
• Reactivities: Human, Mouse, Bovine, Rat
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide (C-MSIRVTQKSYKVST) of Human keratin K8 (formerly also designated cytokeratin 8), coupled to KLH.
• Specificity: Specific for Keratin K8. Marker for discrimination of simple / non-stratified epithelia from those of stratified origin.
  Basic Human keratin K8 (Mr 52 000) of all simple type and complex epithelia.
• Formulation: State: Serum
  State: Liquid Stabilized Serum
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3856 Human
  UniProt ID P05787
• Background: Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
• Synonyms: KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A