Cytokeratin 1 (KRT1) Guinea Pig Polyclonal Antibody

CAT#: BP5071

Cytokeratin 1 (KRT1) guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Cytological Material.
  Western blotting: 1/3,000 (ECL).
  Immunohistochemistry on Frozen Sections: 1/200.
  Immunohistochemistry on Paraffin Embedded Material: 1/100 (after microwave treatment or trypsin).
  Incubation Time: 1 h at RT, extended with paraffin sections.
  Positive Control: Human foot sole.
• Reactivities: Human, Mouse
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of Human keratin K1 (formerly also designated Cytokeratin 1) coupled to KLH
• Specificity: Reacts with Basic keratin K1 (Mr 66 000).
  Specific for keratin K1 in suprabasal layers of cornified squamous epithelia, e.g. the suprabasal layer of scalp and foot sole.
  Negative with non-keratinizing stratified and non-stratified epithelia (e.g. esophagus, duodenum)!
• Formulation: State: Serum
  State: Liquid antiserum containing 0.09% Sodium Azide and 0.5% BSA as stabilizer.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: keratin 1
• Database Link:
  Entrez Gene 3848 Human
  UniProt ID P04264
• Background: Cytokeratin 1 is a type II Cytokeratin. The type II Cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II Cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.
• Synonyms: Keratin-1, KRT1, K1, Cytokeratin 1, Hair alpha protein, 67 kDA cytokeratin, KRTA