Cingulin (CGN) Guinea Pig Polyclonal Antibody

SKU BP5026

Cingulin (CGN) guinea pig polyclonal antibody, Serum

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: Immunoblotting.; Immunofluorescence Microscopy: 1/100. Dilute immedialtely with PBS before use.; Immunohistochemistry on Frozen Sections On cultured cells initial fixation with formaldehyde is recommended for optimal staining.; Immunohistochemistry on Paraffin Sections (Only after microwave treatment).; Positive Control: Total cell lysate of e.g. CaCo-2 cells.
• Reactivity: Bovine, Human, Mouse, Rabbit, Xenopus
• Antibody Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Full length GST-fusion protein of Human Cingulin
• Specificity: The antibody reacts specifically with cingulin (a ca.150 kD polypeptide) present in tight junctions (zonula occludens) of polar epithelia (e.g. colon, duodenum, kidney, liver, pancreas) of transitional epithelia (e.g. bladder epithelium) and of stratified epithelia (e.g. skin, foot sole, tongue, oesophagus, vagina). It is negative with endothelia of vessels and capillaries (e.g. in heart and brain).; Tested Reactivities on Cultured Cell Lines: Carcinoma cell lines of Human (e.g. MCF-7, Caco-2, PLC, A-431), Bovine (MDBK, BMGE) and Canine (MDCK) origin.
• Buffer: State: Serum; State: Liquid Stabilized Antiserum with 0.5% BSA and 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: cingulin
• Database Link:
  Q9P2M7
• Background: Cingulin, a polypeptide of 126.5 kD (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5 (very similar to that of symplekin, another junctional protein)
• Synonyms: CGN, KIAA1319

Reference Data

• Protein Pathways: Tight junction