Cytokeratin 14 (KRT14) Guinea Pig Polyclonal Antibody
Cytokeratin 14 (KRT14) guinea pig polyclonal antibody, Serum
|Recommended Dilution||Western Blot: 1/5000-1/10000.
Immunohistochemistry on Frozen Sections: 1/100-1/200.
Immunohistochemistry on Paraffin Sections: 1/50 (microwave treatment recommended).
Incubation Time: 1 h at RT, extended with Paraffin.
|Reactivities||Bovine, Human, Mouse|
|Immunogen||Peptide against Human K14 C-VSTHEQVLRTKN (460-472)|
|Specificity||The antiserum reacts with keratin K14, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands. In several studies the correlation between the expression of keratin K14 in different types of carcinomas (putatively derived from basal epithelial cells) and prognosis has been discussed (see references listed below).
Reactive Polypeptide: Acidic keratin K14 (Mr 50 000; formerly also designated cytokeratin 14), expressed in basal and first suprabasal layers of epidermis.
Tested cultured cell lines: BPH-1 (derived from non-neoplastic prostatic tissue).
State: Liquid stabilized antiserum
Stabilizer: 0.5% BSA
Preservative: 0.09% Sodium Azide
|Storage||Store undiluted at 2-8°C.|
|Stability||Shelf life: one year from despatch.|
|Background||Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed in pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The α-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and in addition they aid in the characterization of malignant tumors. In Bowen’s disease, the characteristic malignancy of the epidermis exhibits distinct expression patterns of Cytokeratin 14. Mutations in the gene encoding human Cytokeratin 14 lead to epidermolysis bullosa simplex, an inheritied skin disorder characterized by skin blistering due to basal keratinocyte fragility.
Acidic keratin K14 (Mr 50 000; formerly also designated cytokeratin 14), expressed in basal and first suprabasal layers of epidermis.
|Synonyms||Cytokeratin-14, CK14, Keratin 14, K14, KRT14|
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