beta 2 Microglobulin (B2M) Mouse Monoclonal Antibody [Clone ID: B2M-01]

CAT#: BM604HRP

beta 2 Microglobulin (B2M) mouse monoclonal antibody, clone B2M-01, HRP

Product Images

Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-Human β2-microglobulin antibody. Lane 1: RAJI Human Burkitt lymphoma cell line. Lane 2: EL4 Mouse lymphoblastic lymphoma cell line. Lane 3: U937 Human histiocytic lymphoma cell line.

Specifications

Product Data

• Clone Name: B2M-01
• Applications: ELISA, WB
• Recommended Dilution: The antibody is designed for quantitative determination of soluble beta 2-Microglobulin/MHC Class I complexes. This antibody can also be used for detection of free beta 2-Microglobulin by Western blotting.
  ELISA.
  Western blot (Both Reducing and non-Reducing conditions):
  Recommended Dilution: 2-4 µg/ml.
  Positive Control: U937 Human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line.
  Negative Control: EL4 mouse lymphoblastic lymphoma cell line.
  Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified Human beta-2-Microglobulin
• Specificity: The antibody reacts with beta-2-Microglobulin (B2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta-2-Microglobulin.
  Negative Species: Mouse, Bovine, Canine (Dog), Rabbit and Chicken.
• Formulation: PBS
  Label: HRP
  State: Liquid purified Ig fraction
  Preservative: 0.01% (w/v) Thimerosal
  Label: Conjugated with Horseradish Peroxidase of high specific activity and RZ=3.
  The reagent is free of unconjugated as determined by size-exclusion chromatography.
• Concentration: lot specific
• Conjugation: HRP
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
  Should it contain a precipitate we recommend microcentrifugation before use.
• Stability: Shelf life: one year from despatch.
• Gene Name: beta-2-microglobulin
• Database Link:
  Entrez Gene 567 Human
  UniProt ID P61769
• Background: b2M non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
• Synonyms: B2M, Beta-2 microglobulin, Beta 2 microglobulin