beta 2 Microglobulin (B2M) Mouse Monoclonal Antibody [Clone ID: B2M-01]

CAT#: BM604B

beta 2 Microglobulin (B2M) mouse monoclonal antibody, clone B2M-01, Biotin

Product Images

Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) PE.

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  Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) FITC.

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  Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human β2-microglobulin (B2M-01). Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line

Specifications

Product Data

• Clone Name: B2M-01
• Applications: FC
• Recommended Dilution:

  Biotinylated antibody is designed for Indirect Immunofluorescence analysis by Flow Cytometry.
  Recommended Working Dilutions: 1/200 as a starting point.

• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified Human Beta-2-microglobulin
• Specificity: The antibody reacts with Beta-2-microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble Beta-2-microglobulin.
• Formulation: Phosphate buffered saline (PBS), pH~7.4
  Label: Biotin
  State: Liquid purified Ig fraction
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Concentration: lot specific
• Conjugation: Biotin
• Storage:

  Store undiluted at 2-8°C.
  DO NOT FREEZE!
  

• Stability: Shelf life: one year from despatch.

• Gene Name: beta-2-microglobulin
• Database Link:
  Entrez Gene 567 Human
  UniProt ID P61769
• Background: b2M is a 12 kDa Ig like glycoprotein. It non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
• Synonyms: B2M, Beta-2 microglobulin, Beta 2 microglobulin