OATP2 (SLCO1B1) (+ OATP8) Mouse Monoclonal Antibody [Clone ID: mMDQ]

SKU BM5541

OATP2 (SLCO1B1) (+ OATP8) mouse monoclonal antibody, clone mMDQ, Supernatant

Product Data

• Clone Name: mMDQ
• Application: IF, IHC, IP, WB
• Recommended Dilution: Immunoprecipitation.; Immunofluorescence Microscopy.; Western blotting: For optimal detection of the glycosylated antigen sample boiling before SDS-PAGE is not recommended. Alternatively, samples can be kept at 37°C for 30 min (See also Ref.1).; Immunohistochemistry on Paraffin Sections (Only after microwave treatment): Use undiluted.; For details See Ref. 1.; Incubation Time: 1 h at RT, for Paraffin Sections overnight at 2-8°C.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic N-terminus (24 aa) of Human organic anion transporter OATP2 coupled to KLH
• Specificity: In Western blot analyses of basolateral membrane fractions of human liver the monoclonal antibody mMDQ reacts with 90 kD and 120 kD proteins (after SDS-PAGE) representing OATP2 (also described as OATP-C) and OATP8, respectively. According to a recent new nomenclature these hepatocyte-specific proteins are identical to OATP1B1 and OATP1B3, respectively (See Ref.2).; Using immunofluorescence microscopy mMDQ stains the sinusoidal (basolateral) membranes in frozen sections of human liver, hepatocellular carcinoma (See Ref.1).; Reacts with OATP2 and OATP8, human organic anion transporters (90 kD, 120 kD, after SDS-PAGE).; Reacts with Transfected HEK293 cells; negative with HepG2 and PLC.
• Buffer: State: Supernatant; State: Liquid Hybridoma Culture Supernatant; Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: solute carrier organic anion transporter family member 1B1
• Database Link:
  Q9Y6L6
• Synonyms: LST1, LST-1, OATP-2, OATPC, OATP-C, SLC21A6
• Note: Protocol: Western Blotting (Ref.1); 1. For optimal detection of the glycosylated antigen sample boiling before SDS-PAGE is not recommended; alternatively, samples can be kept at 37°C for 30 min; 2.50 µg of protein lysate should be loaded on each lane.; 3. The usage of a PVDF-Membrane is recommended.; 4. The usage of 10% milk powder in TTBS (Tris-buffered saline, pH 7.6, with 0.05% Tween 20) as blocking reagent. The antibody can be diluted 3-5 x

Reference Data

• Protein Families: Druggable Genome, Transmembrane