ACTC1 Mouse Monoclonal Antibody [Clone ID: AC1-20.4.2]

SKU BM5079

ACTC1 mouse monoclonal antibody, clone AC1-20.4.2, Purified

Product Data

• Clone Name: AC1-20.4.2
• Application: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/500.; Western Blot: 1/1000 (using the ECL-enhanced procedure).; Immunohistochemistry on Frozen Sections: 1/10.; Immunohistochemistry on Paraffin-Embedded Tissue: 1/10 (After microwave treatment).; Incubation time: 1 h at RT.; Special Treatment: It is necessary to include 0.5 M NaCl in all washing buffers for application on Frozen tissue and 1 M NaCl for Western blotting and ELISA to enhance specifity. This is not necessary for Paraffin sections.
• Reactivity: Bovine, Chicken, Human, Rabbit
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic NH2 terminus decapeptide of cardiac isoform of Actin.
• Specificity: This antibody is specific for Fetal (Cardiac) isoform of Actin.; Clone Ac1 represents an excellent marker for cardiac tissue. It discriminates fetal (cardiac) alpha-Actin from all other Actin isoforms.; Mab Ac1-20.4.2 shows no cross reaction with other Actin isoforms present in skeletal and smooth muscle, provided that stringent conditions have been applied (see Special Treatment).
• Buffer: Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer.; State: Purified; State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in 1.0 ml distilled water.
• Purification: Affinity Chromatographyon Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody After reconstitution at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: actin, alpha, cardiac muscle 1
• Database Link:
  P68032
• Background: Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).; Fetal Actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord.
• Synonyms: ACTC; ASD5; CMD1R; CMH11

Reference Data