Surfactant protein D (SFTPD) (Conformational epitope) Mouse Monoclonal Antibody [Clone ID: IIE11 (SPDE)]

SKU BM4083

Surfactant protein D (SFTPD) (Conformational epitope) mouse monoclonal antibody, clone IIE11 (SPDE), Purified

Product Data

• Clone Name: IIE11 (SPDE)
• Application: ELISA, IHC, WB
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 2 µg/ml (1/250); Does not react on routinely processed paraffin sections.; Recommneded Positive Control: Rat lung.; Has been described to work in Western blots.
• Reactivity: Human, Rat
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified Rat Surfactant protein D from bronchoalveolar lavage.
• Specificity: SPDE recognizes a conformational epitope of SP-D.; Clone SPDE is a reclone from clone IIE11, with identical reactivity.; Antigen Distribution on Tissue Sections: Alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits.; Alveolar type III cells stain negative.; No reaction on rat skin, colon, kidney and liver.
• Buffer: Stock solution contains PBS, pH 7.2 with 0.09% Sodium Azide as preservative and 5 mg/ml BSA as stabilizer.; State: Purified; State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore by adding 0.5 ml distilled water (= 0.5 mg/ml Stock Solution).
• Concentration: 0.5 mg/ml
• Purification: Affinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store original vial at 2-8°C and stock solution or aliquots thereof at -20°C.; Avoid repeated thawing and freezing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Gene Name: surfactant protein D
• Database Link:
  P35247
• Background: Surfactant Protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43kDa in its reduced state, 620kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.
• Synonyms: Lung surfactant protein D, SP-D, PSP-D, PSPD, SFTP4, Collectin-7, COLEC7

Reference Data