CD46 Mouse Monoclonal Antibody [Clone ID: 13/42]

CAT#: BM4042

CD46 mouse monoclonal antibody, clone 13/42, Purified

Product Images

Staining of Human Tonsil Frozen Section using CD46 Antibody (Clone 13/42)

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  Staining of Human Placenta Frozen Section using CD46 Antibody (Clone 13/42)

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  Table 1. Tissue distribution of CD46 (Johnstone et al., modified)

Specifications

Product Data

• Clone Name: 13/42
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 5-10 µg/ml of neat antibody to label 1x10⁶ cells.
  Immunoprecipitation: 5 µg/200 µl extract from 2x10⁶ cells.
  Immunohistochemistry on Frozen Sections: 1-2 µg/ml (1/200-1/400).
  Immunohistochemistry on Paraffin Sections: 8 µg/ml (1/50).
  Proteinase K pretreatment for antigen retrieval is recommended.
  Suggested Positive Control: Human tonsil.
  Has been described to work in FACS, Western Blots and Functional Studies.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human U-251 cells.
  Epitope: Localized in the first two short consensus repeats.
• Specificity: The monoclonal antibody 13/42 recognizes Human CD46.
  The recognized epitope is localized on the first two short consensus repeats.
  Cross-reacts with African green monkey.
  Antigen Distribution: The antigen is widely expressed on Human cells except erythrocytes. It can also be found as a soluble protein in serum and saliva. Many Human cell lines express CD 46 e.g. U937, HeLa.
• Formulation: PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a preservative
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: 0.4 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD46 molecule
• Database Link:
  Entrez Gene 4179 Human
  UniProt ID P15529
• Background: CD46 also known as Membrane Cofactor Protein (MCP), acts as a cofactor for factor I, a serine protease which is involved in the degradative cleavage of C3b and C4b. Structurally as well as functionally, it is a member of the regulator of complement activation proteins. CD46 is composed of an amino terminus of four short consensus repeating units (SCR), a Ser/Thr (ST)-rich domain, 13 amino acid residues with unknown function, a transmembrane region, and a cytoplasmic tail. There are many isoforms on human cells and in body fluids which are distinguishable by SDS-PAGE. Their heterogeneity stems from varying amounts of O-linked sugars secondary to alternative splicing of mRNA coding the ST-rich region.
• Synonyms: TLX, MIC10
• Note: Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
  The whole procedure is performed at room temperature.
  1. Wash in PBS.
  2. Block endogenous peroxidase.
  3. Wash in PBS.
  4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber.
  5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber.
  6. Wash in PBS.
  7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber.
  8. Wash in PBS.
  9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  10. Wash in PBS.
  11. Counterstain with Mayer's hemalum.
  
  Protocol with formalin-fixed, paraffin-embedded sections:
  The whole procedure is performed at room temperature.
  1. Deparaffinize and rehydrate tissue section.
  2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS.
  6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber.
  7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber.
  8. Wash in PBS.
  9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber.
  10. Wash in PBS.
  11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  12. Wash in PBS.
  13. Counterstain with Mayer's hemalum.