S100A9 Mouse Monoclonal Antibody [Clone ID: S36.48]

SKU BM4026B

S100A9 mouse monoclonal antibody, clone S36.48, Biotin

Product Data

• Clone Name: S36.48
• Application: ELISA, FC, IHC
• Recommended Dilution: ELISA.; Immunohistochemistry on Frozen Sections: 1 µg/ml (1/200).; Immunohistochemistry on Paraffin Sections: 2 µg/ml (1/100). Proteinase K pretreatment for antigen retrieval is recommended.; Suggested Positive Control: Human tonsil.; Also Suitable for Dot Blots and FACS.
• Reactivity: Bovine, Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cultured Human monocytes.
• Specificity: MRP14, granulocytes, stimulated monocytes and macrophages.; Monoclonal antibody BM4026B identifies the Ca2+-binding 14 kD subunit of the inflammatory L-1 protein complex, also called S100A9 or Calgranulin B. It is useful for the characterization of circulating granulocytes or inflammatory infiltrates of the myelo-monocytic lineage which express MRP14 differently depending on the inflammatory status of the disease.; Antigen Distribution; Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from all other blood cells. In cultured monocytes, maximum MRP14 expression is found after 3-4 days. Myeloid leukaemic cells have been found to be positive as well.; Tissue Sections: MRP14 is found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP14 increasingly during the early stages of inflammation. A high MRP14 (and low MRP8) expression by macrophages was reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases like chronic rheumatoid arthritis, MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8/14 determined by antibody 27E10 (product Cat.-No BM4025).
• Buffer: PBS, pH 7.2; Label: Biotin; State: Lyophilized purified IgG fraction; Stabilizer: 5 mg/ml BSA; Preservative: 0.05% (v/v) Kathon CG
• Reconstitution Method: Restore by adding 0.5 ml distilled water.
• Concentration: 0.2 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Gene Name: S100 calcium binding protein A9
• Database Link:
  P06702
• Background: S100A9 is a member of the S100 family of proteins. S100A9, together with S100A8 forms a heterodimeric protein complex, Calprotectin, which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. Complexes of S100A8 and S100A9 are the physiologically relevant forms of these proteins.; S100A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. Its expression and potential cytokine-like function in inflammation and in cancer suggest that S100A8/A9 may play a key role in inflammation-associated cancer.
• Synonyms: S100-A9, CAGB, MRP-14

Reference Data