Adgre1 Rat Monoclonal Antibody [Clone ID: CI:A3-1]

SKU BM4008F

Adgre1 rat monoclonal antibody, clone CI:A3-1, FITC

Product Data

• Clone Name: CI:A3-1
• Application: FC, IF
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10⁶ cells in 100 µl. ; Immunofluorescence.
• Reactivity: Mouse
• Antibody Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice.; Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS-1 myeloma cell line.
• Specificity: This antibody recognises the F4/80 antigen.; Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes. We recommend the use of BM4008LE for this purpose.
• Buffer: PBS, pH 7.4; Label: FITC; State: Liquid purified IgG fraction; Stabilizer: 1% BSA; Preservative: 0.09% Sodium Azide; Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; This product is photosensitive and should be protected from light.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: adhesion G protein-coupled receptor E1
• Database Link:
  Q61549
• Background: F4/80 is a 160 kD cell surface glycoprotein that is a member of the EGFTM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucinlike hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
• Synonyms: Emr1, Gpf480

Reference Data