Cd68 Mouse Monoclonal Antibody [Clone ID: ED1]

CAT#: BM4000

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Cd68 mouse monoclonal antibody, clone ED1, Purified

Size: 100 ug 250 ug

Conjugation: Unconjugated Biotin FITC



USD 765.00

5 Days*

Size
    • 250 ug

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Specifications

Product Data
Clone Name ED1
Applications FC, IF, IHC, IP, R, WB
Recommended Dilution RIA.
Western blotting.
Immunoprecipitation.
Immunofluorescence.
Flow Cytometry:
Use 10 µl of 1/25-1/100 diluted CD68 antibody to label 106 cells in 100 µl (Membrane permeabilisation is required for best results).
Immunohistology on Frozen Sections.
Immunohistology on Paraffin Sections: 
1/50-1/100. Requires protein digestion pretreatment of Paraffin sections with Pronase or Trypsin.
Reactivities Bovine, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Rat spleen cells.
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
Specificity CD68 Antibody (Cat#BM4000) recognises a single chain glycoprotein of 110 kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes.
Formulation PBS, pH 7.4 containing 0.09% Sodium Azide as preservative
State: Purified
State: Liquid purified IgG fraction
Concentration lot specific
Purification Affinity Chromatography on Protein G
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life one year from despatch.
Background The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
Synonyms Gp110, Macrosialin, Macrophage marker
Note Studies have shown that the antigen recognised by ED1 is the Rat homologue of Human CD68.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.