Emcn Rabbit Polyclonal Antibody
Product Data | |
Application | FC, IF, IHC, WB |
---|---|
Recommended Dilution | Western blot: 1-5 μg/ml. FACS: 1-5 µg/ml. Immunofluorescence/Immunohistochemistry on Frozen Sections: 5 μg/ml. |
Reactivity | Mouse |
Antibody Host | Rabbit |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Highly pure (>95%) recombinant mouse soluble Endomucin (Glu20-Ser190) derived from E. coli (Cat.-No. AR60010PU-N) |
Specificity | This antibody detects Mouse Endomucin (Glu20-Ser190). Other species not tested. |
Buffer | PBS State: Aff - Purified State: Lyophilized purified Ig fraction |
Reconstitution Method | Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Purification | Antigen Affinity Chromatography |
Conjugation | Unconjugated |
Storage | Store lyophilized at 2-8°C for 6 month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Shipping | Ambient |
Gene Name | endomucin |
Database Link | |
Background | Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80 - 120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21 - 190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91 - 141, a second shows a one aa substitution for aa 91 - 129, and a third shows a one aa substitution for aa 129 - 142. Over aa 21 - 90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively. |
Synonyms | EMCN, EMCN2, MUC14, Endomucin-2, Mucin-14, Gastric cancer antigen Ga34 |
Reference Data |
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