Emcn Rabbit Polyclonal Antibody

CAT#: AP60010PU-S

Emcn rabbit polyclonal antibody, Aff - Purified

Product Images

Western analysis using anti-mouse Endomucin antibody. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa). Lane 2: rm sEndomucin (250ng).

• 

  Immunofluorescence staining (green) of cryo-sections of adult mouse kidney (fixed 15 min in 4% PFA) with anti-mouse Endomucin antibody. (5ug/ml) and counter staining of nuclei with DAPI (blue). <i>Performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.</i>

• 

  FACS analysis with primary mouse endothelial cells (SnoMec).

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: Western blot: 1-5 μg/ml.
  FACS: 1-5 µg/ml.
  Immunofluorescence/Immunohistochemistry on Frozen Sections: 5 μg/ml.
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Highly pure (>95%) recombinant mouse soluble Endomucin (Glu20-Ser190) derived from E. coli (Cat.-No. AR60010PU-N)
• Specificity: This antibody detects Mouse Endomucin (Glu20-Ser190).
  Other species not tested.
• Formulation: PBS
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Antigen Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 month or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: endomucin
• Database Link:
  Entrez Gene 59308 Mouse
  UniProt ID Q9R0H2
• Background: Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80 - 120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21 - 190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91 - 141, a second shows a one aa substitution for aa 91 - 129, and a third shows a one aa substitution for aa 129 - 142. Over aa 21 - 90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively.
• Synonyms: EMCN, EMCN2, MUC14, Endomucin-2, Mucin-14, Gastric cancer antigen Ga34