EGFL7 (C-term) Rabbit Polyclonal Antibody

SKU AP60007PU-L

EGFL7 (C-term) rabbit polyclonal antibody, Purified

Product Data

• Application: IF, IHC, IP, WB
• Recommended Dilution: Western blot: 2-5 µg/ml.; Immunohistochemistry on Frozen sections. ; Immnunocytochemistry.; Immunoprecipitation.
• Reactivity: Human
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Highly pure recombinant human EGFL7 (Pro178-Ser273) C-terminus derived from insect cells
• Specificity: This antibody recognizes VE-Statin / EGFL7 at C-term.
• Buffer: PBS; State: Purified; State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in sterile water/PBS to a concentration of 0.1-1.0 mg/ml.
• Purification: Protein A chromatography
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at 2-8°C.; Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Gene Name: EGF like domain multiple 7
• Database Link:
  Q9UHF1
• Background: EGFL7 (EGF-like domain-containing protein 7) regulates vascular tubulogenesis in vivo. During sprouting angiogenesis, groups of endothelial cells (ECs) migrate together in units called sprouts. EGFL7 regulates the proper spatial organization of ECs within each sprout and influences their collective movement. It inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate in vitro.; EGFL7 (VE-Statin) is an ∼ 30 kDa secreted protein that contains an Emilin-like (EMI) domain (a multimerization motif), and two epidermal growth factor (EGF) domains, one of which binds calcium. Based on these domains, it has been hypothesized that EGFL7 may self-assemble like extracellular matrix (ECM) proteins and, thus, could incorporate into ECM. EGFL7 has been reported to stimulate cell adhesion as well as motility in a manner similar to ECM proteins. EGFL7 has been shown to be primarily expressed by developing ECs but also by primordial germ cells and some central nervous system neurons. Interestingly, EGFL7 expression markedly decreases in ECs in postnatal life, but can be strongly up-regulated after various tissue injuries that lead to increased angiogenic responses.
• Synonyms: EGF-like protein 7, EGF like protein 7, VE-statin, NOTCH4-like protein, Zneu1, MEGF7, UNQ187, PRO1449

Reference Data