Phospholipase D2 (PLD2) pTyr169 Rabbit Polyclonal Antibody

CAT#: AP55830PU-S

Phospholipase D2 (PLD2) pTyr169 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from Jurkat cells treated with TNF using PLD2 (Phospho-Tyr169) Antibody.The lane on the right is treated with the antigen-specific peptide.

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  Immunohistochemical analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) Antibody (left)or the same antibody preincubated with blocking peptide (right).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1:500~1:1000.
  Immunohistochemistry on paraffin sections: 1:50~1:100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 169(E-N-Y(p)-L-N) derived from Human PLD2 (KLH-conjugated)
• Specificity: The antibody detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 95 kDa
• Gene Name: phospholipase D2
• Database Link:
  Entrez Gene 5338 Human
  UniProt ID O14939
• Background: Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.
• Synonyms: Phospholipase D2, PLD 2, Choline phosphatase 2, PLD1C, Phosphatidylcholine-hydrolyzing phospholipase D2