ZBTB4 Rabbit Polyclonal Antibody

CAT#: AP55402PU-N

ZBTB4 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot: 1/500-1/5000.
  Immunohistochemistry: 1/100-1/500.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide derived from an internal domain of Human ZBTB4
• Specificity: This antibody reacts with 105 kDa ZBTB4 protein.
• Formulation: 0.1M Tris, 0.1M Glycine, 2% Sucrose
  State: Purified
  State: Lyophilized purified powder
  Preservative: None
• Reconstitution Method: Restore in distilled water.
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: zinc finger and BTB domain containing 4
• Database Link:
  Entrez Gene 57659 Human
  UniProt ID Q9P1Z0
• Background: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions; this degradation increases with DNA damage.
• Synonyms: KIAA1538, KAISO-L1