SIAH2 Rabbit Polyclonal Antibody

SKU AP54906SU-N

SIAH2 rabbit polyclonal antibody, Serum

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: ELISA. ; Western Blot: 1/500-1/2 000; Immunohistochemistry: 1/100-1/500.
• Reactivity: Human
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide derived from N-terminal region of SIAH-2
• Specificity: Reacts with Human 35 kDa Siah-2. ; Cross reacts with all species due to sequence homolgy.
• Buffer: State: Serum; State: Lyophilized powder; Preservative: None
• Reconstitution Method: Restore in distilled water.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Gene Name: siah E3 ubiquitin protein ligase 2
• Database Link:
  O43255
• Background: SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkappaB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.
• Synonyms: Siah-2

Reference Data