TPK1 (N-term) Rabbit Polyclonal Antibody

CAT#: AP54344PU-N

TPK1 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of TPK1 Antibody (N-term) in mouse spleen tissue lysates (35 ug/lane). TPK1 (arrow) was detected using the purified Pab.

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  Western blot analysis of TPK1 (arrow) using rabbit polyclonal TPK1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPK1 gene.

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  Formalin-fixed and paraffin-embedded human testis tissue reacted with TPK1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected from the N-terminal region of Human TPK1
• Specificity: This antibody detects Human and Mouse Thiamine pyrophosphokinase 1 (TPK1) (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: thiamin pyrophosphokinase 1
• Database Link:
  Entrez Gene 29807 MouseEntrez Gene 27010 Human
  UniProt ID Q9H3S4
• Background: TPK1 is a protein, that exists as a homodimer, which catalyzes the conversion of thiamine to thiamine pyrophosphate.
• Synonyms: hTPK1; PP20
• Note: Molecular Weight: 27265 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Thiamine metabolism