SF3B2 (Center) Rabbit Polyclonal Antibody

CAT#: AP53869PU-N

SF3B2 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis using SF3B2 Antibody (Center) in A549 cell line lysates (35ug/lane). This demonstrates the SF3B2 antibody detected the SF3B2 protein (arrow).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100-1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 515-545 amino acids from the Central region of Human SF3B2
• Specificity: Recognizes SF3B2 (Center).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide Affinity purification
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: splicing factor 3b subunit 2
• Database Link:
  Entrez Gene 10992 Human
  UniProt ID Q13435
• Background: This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. [provided by RefSeq].
• Synonyms: Splicing factor 3B subunit 2,SAP145
• Note: Molecular Weight: 100228 Da

Reference Data

• Protein Pathways: Spliceosome