RPB11 (POLR2J) (C-term) Rabbit Polyclonal Antibody

CAT#: AP53387PU-N

RPB11 (POLR2J) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of POLR2J Antibody (C-Term) in Jurkat cell line lysates (35ug/lane). This demonstrates the POLR2J antibody detected the POLR2J protein (arrow).

• 

  Western blot analysis of POLR2J Antibody (C-Term) in Mouse Neuro-2a cell line lysates (35ug/lane). This demonstrates the POLR2J antibody detected the POLR2J protein (arrow).

• 

  Formalin fixed, paraffin embedded human skeletal muscle stained with POLR2J Antibody (C-Term) followed by peroxidase conjugation of the secondary antibody and DAB staining

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 93-117 amino acids from the C-terminal region of Human POLR2J.
• Specificity: This antibody recognizes Human and Mouse POLR2J (C-Term).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: polymerase (RNA) II subunit J
• Database Link:
  Entrez Gene 20022 MouseEntrez Gene 5439 Human
  UniProt ID P52435
• Background: This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.
• Synonyms: RNA polymerase II subunit B11-a, POLR2J1, RPB11a
• Note: Molecular Weight: 13293 Da

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase