PDHA2 (Center) Rabbit Polyclonal Antibody

CAT#: AP53242PU-N

PDHA2 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PDHA2 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). This demonstrates the PDHA2 antibody detected the PDHA2 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue reacted with PDHA2 Antibody (Center) followed by peroxidase conjugation of the secondary antibody and DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 290-318 amino acids from the Central region of Human PDHA2
• Specificity: This antibody recognizes Human PDHA2 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: pyruvate dehydrogenase (lipoamide) alpha 2
• Database Link:
  Entrez Gene 5161 Human
  UniProt ID P29803
• Background: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
• Synonyms: PDHAL, PDHE1-A type II
• Note: Molecular Weight: 42933 Da

Reference Data

• Protein Pathways: Butanoate metabolism, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine biosynthesis