Oxytocin neurophysin 1 (OXT) (Center) Rabbit Polyclonal Antibody

CAT#: AP52854PU-N

Oxytocin neurophysin 1 (OXT) (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of Oxytocin Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue reacted with Oxytocin Antibody (Center), which was peroxidase conjugated to the secondary antibody and followed by DAB staining.

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  Flow cytometric analysis of MDA-MB231 cells using Oxytocin Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: >1/1000 (Peptide Elisa only)
  Western Blot: 1/1,000.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 11~40 amino acids from the Central region of Human Ocytocin
• Specificity: This antibody recognizes Human Ocytocin (Center). Other species not tested.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: oxytocin/neurophysin I prepropeptide
• Database Link:
  Entrez Gene 5020 Human
  UniProt ID P01178
• Background: There are two proteins encoded by this gene, oxytocin and neurophysin I. Oxytocin is posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. The precursor seems to be activated while it is being transported along the axon to the posterior pituitary. This hormone contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.
• Synonyms: OXT, Ocytocin
• Note: Molecular Weight: 12722 Da

Reference Data

• Protein Families: Secreted Protein