Midkine (MDK) (C-term) Rabbit Polyclonal Antibody

CAT#: AP52643PU-N

Midkine (MDK) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of Midkine Antibody (C-term) in mouse kidney tissue lysates (35ug/lane).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue reacted with Midkine Antibody (C-term), which was peroxidase conjugated to the secondary antibody and followed by DAB staining.

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  Confocal immunofluorescent analysis of Midkine Antibody (C-term) with Hela cell followed by Alexa Fluor®488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  Confocal immunofluorescent analysis of Midkine Antibody (C-term) with HepG2 cell followed by Alexa Fluor®488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 113-143 amino acids from the C-terminal region of Human Midkine
• Specificity: This antibody recognizes Human and Mouse Midkine (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: midkine (neurite growth-promoting factor 2)
• Database Link:
  Entrez Gene 17242 MouseEntrez Gene 4192 Human
  UniProt ID P21741
• Background: Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis (summary by Reynolds et al., 2004 [PubMed 15197188]).
• Synonyms: MK, MDK, MK1, NEGF2, Neurite outgrowth-promoting protein
• Note: Molecular Weight: 15585 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane