Leucyl tRNA synthetase (LARS) (N-term) Rabbit Polyclonal Antibody

CAT#: AP52444PU-N

Leucyl tRNA synthetase (LARS) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of LARS Antibody (N-term) in mouse Neuro-2a cell line lysates (35ug/lane). This demonstrates the LARS antibody detected the LARS protein (arrow).

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  Flow cytometric analysis of Hela cells using LARS Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 183-213 amino acids from the N-terminal region of Human LARS.
• Specificity: This antibody recognizes Human and Mouse LARS (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: leucyl-tRNA synthetase
• Database Link:
  Entrez Gene 51520 Human
  UniProt ID Q9P2J5
• Background: This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. Alternatively spliced transcript variants of this gene have been found; however, their full-length nature is not known.
• Synonyms: LARS, KIAA1352, Leucyl-tRNA synthetase, cytoplasmic, EC=6.1.1.4, Leucine-tRNA ligase, LeuRS
• Note: Molecular Weight: 134466 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis