ID4 (N-term) Rabbit Polyclonal Antibody

CAT#: AP52145PU-N

ID4 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ID4 Antibody (N-term) in Mouse bladder tissue lysates (35ug/lane). ID4 (arrow) was detected using the purified Pab.

• 

  Immunohistochemistry analysis in formalin fixed and paraffin embedded bladder carcinoma reated with ID4 Antibody (N-term), which was peroxidase conjugated to the secondary antibody and followed by DAB staining.

• 

  Flow cytometric analysis of 293 cells using ID4 Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of Human ID4 / BHLHB27
• Specificity: This antibody recognizes Human and Mouse ID4 / BHLHB27 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: inhibitor of DNA binding 4, HLH protein
• Database Link:
  Entrez Gene 15904 MouseEntrez Gene 3400 Human
  UniProt ID P47928
• Background: ID4 is transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins.
• Synonyms: DNA-binding protein inhibitor ID-4, Inhibitor of DNA binding 4
• Note: Molecular Weight: 16622 Da

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway