HLA-DQA1 (N-term) Rabbit Polyclonal Antibody

CAT#: AP52054PU-N

HLA-DQA1 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of HLA-DQA1 Antibody (N-term) Cat.-No AP52054PU-Nin NCI-H460 cell line lysates (35ug/lane). This demonstrates the HLA-DQA1 antibody detected the HLA-DQA1 protein (arrow).

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  Western blot analysis of HLA-DQA1 (arrow) using HLA-DQA1 Antibody (N-term) Cat.-NoAP52054PU-N.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-DQA1 gene.

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  Flow cytometric analysis of NCI-H460 cells using HLA-DQA1 Antibody (N-term) Cat.-NoAP52054PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 46-74 amino acids from the N-terminal region of human HLA-DQA1
• Specificity: This antibody recognizes Human HLA-DQA1 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  UniProt ID P01908
• Background: HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.
• Synonyms: HLA class II histocompatibility antigen, DQ alpha 1 chain
• Note: Molecular Weight: 27805 Da