Glutathione Reductase (GSR) (C-term) Rabbit Polyclonal Antibody

CAT#: AP51963PU-N

Glutathione Reductase (GSR) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Glutathione reductase Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma reacted with Glutathione reductase Antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Confocal immunofluorescent analysis of Glutathione reductase Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of NCI-H460 cells using Glutathione reductase Antibody (C-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunofluoresecnce: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 387-416 amino acids from the C-terminal region of human Glutathione reductase.
• Specificity: This antibody recognizes Human Glutathione reductase (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: glutathione reductase
• Database Link:
  Entrez Gene 2936 Human
  UniProt ID P00390
• Background: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
• Synonyms: GRase, GR, GSR, GLUR, GRD1
• Note: Molecular Weight: 56257 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism