Dysadherin (FXYD5) (Center) Rabbit Polyclonal Antibody

CAT#: AP51745PU-N

Dysadherin (FXYD5) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Dysadherin / FXYD5 Antibody (Center) in CEM cell line lysates (35ug/lane). This demonstrates the FXYD5 antibody detected the FXYD5 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue reacted with Dysadherin / FXYD5 Antibody (Center) followed by peroxidase conjugation of the secondary antibody and DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Peptide ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 71-100 amino acids from the Central region of Human Dysadherin / FXYD5
• Specificity: This antibody recognizes Human Dysadherin / FXYD5 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: FXYD domain containing ion transport regulator 5
• Database Link:
  Entrez Gene 53827 Human
  UniProt ID Q96DB9
• Background: This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. This gene product, FXYD5, is a glycoprotein that functions in the up-regulation of chemokine production, and it is involved in the reduction of cell adhesion via its ability to down-regulate E-cadherin. It also promotes metastasis, and has been linked to a variety of cancers. Alternative splicing results in multiple transcript variants. [RefSeq curation by Kathleen J. Sweadner, Ph.D., sweadner@helix.mgh.harvard.edu.].
• Synonyms: DYSAD, IWU1
• Note: Molecular Weight: 19472 Da

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane