ETS1 (N-term) Rabbit Polyclonal Antibody

CAT#: AP51470PU-N

ETS1 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ETS1 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). This demonstrates the ETS1 antibody detected the ETS1 protein (arrow).

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  Confocal immunofluorescent analysis of ETS1 Antibody (N-term) with MCF-7 cell followed by Alexa Fluor®488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of Jurkat cells using ETS1 Antibody (N-term) (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 46-74 amino acids from the N-terminal region of Human ETS1
• Specificity: This antibody recognizes Human ETS1 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: ETS proto-oncogene 1, transcription factor
• Database Link:
  Entrez Gene 2113 Human
  UniProt ID P14921
• Background: ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (summary by Dwyer et al., 2007 [PubMed 17986575]).
• Synonyms: Protein C-ets-1, EWSR2, p54
• Note: Molecular Weight: 50408 Da

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Dorso-ventral axis formation, Pathways in cancer, Renal cell carcinoma